Professional Skills
I have extensive experience in project management, biotechnology assessment,and commercialization, with a proven track record in driving success through collaborations with CROs and managing complex R&D projects. My work has focused on early phase preclinical drug development, particularly in the areas of small molecule anti-tumor drugs and neurology medicines, contributing to innovations for NASDAQ-listed startups
Laboratory Skills
I possess a wide range of laboratory skills that are a result of my extensive experience in the Biotechnology industry. These are detailed below:
Molecular Biology
- Molecular cloning from cDNA template by polymerase chain reaction Recombinant plasmid construction
- Site-directed mutagenesis: charge-to-alanine scanning mutagenesis; C-terminal deletion protein mutant
- DNA purification through agarose gel extraction, phenol-chloroform extraction, plasmid mini-prep/maxi –prep
- DNA sequencing (manual/automatic)
Protein Expression and Purification
- E.coli expression system for soluble protein of tagged (His-tagged, GST-tagged, MBP-tagged, thioredoxin-tagged)/non-tagged protein
- Baculovirus expression system from SF 21 cell line
- Refolding of E.coli expressed protein in the form of inclusion bodies: refolding by denaturants; redox system; change in pH
- Protein separation by various chromatographic methods: ion-exchange, affinity resin, gel-filtration, hydrophobic interaction
- Production of intact protein from fusion tag protein by protease
Protein Detection and Analysis
- SDS-PAGE analysis
- Native gel electrophoresis
- Basic operation of mass spectroscopy: Maldi-Tof, LC-Mass spectroscopy
- Light scattering
- Western blot
In vitro Protein Assay
- Yeast-two hybrid screening
- In vitro pull-down assay: immobilization of purified protein on resin by appropriated chemistry method and identification of interacting partners
- Protein cross-linking experiment
- Translation inhibition assay: determination of enzyme activity in affecting protein synthesis in nuclease-untreated rabbit reticulocyte lysate
Present day
Currently, I run a UK-based consultancy, where I advise biotech startups on R&D strategies, grant applications, and business development, helping companies bring innovative solutions to market.